Hello

MINI BINDER

One campaign, from a target structure to numbers that came back from the bench.

PD-L1/Campaign/PDL1-R1

The target

PD-L1

Gene: CD274
Organism: Homo sapiens
UniProt: Q9NZQ7
PDB: 4ZQK
Chain: A
Domain: 19-127

The GFCC′ face of the IgV domain — where PD-1 binds, overlapping the atezolizumab epitope. Blocking this face is the campaign's goal.

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The epitope

5 residues to block

A54A56A115A121A123

The GFCC′ face of the IgV domain — where PD-1 binds, overlapping the atezolizumab epitope. Blocking this face is the campaign's goal.

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A binder

DEMO-PDL1-005

MAEEIKAYLQEAKKLGTPSVEEFKKLVKEAEKLLSGGPREEFKKLLEEAKKLLE

54aa

0.848i_ptm

0.87plddt_binder

8.661pae_interaction

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In silico triage

246

Saved numeric cuts, applied to the whole set.

bindcraft_default v1

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The bottleneck

Complex pLDDT

Complex pLDDT 16
i_pAE (normalized) 15
Unsatisfied H-bonds 14
i_pTM 11
Interface H-bonds 11
Shape complementarity 9
Surface hydrophobicity 9

Cut · Not measured · of 24

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Shortlisted

6 made the cut

DEMO-PDL1-005 i_pTM 0.848 · 54 aa Promoted

DEMO-PDL1-004 i_pTM 0.843 · 57 aa Promoted

DEMO-PDL1-001 i_pTM 0.819 · 58 aa Promoted

DEMO-PDL1-003 i_pTM 0.808 · 62 aa Promoted

DEMO-PDL1-006 i_pTM 0.787 · 55 aa Promoted

DEMO-PDL1-002 i_pTM 0.785 · 58 aa Promoted

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Back from the bench

23 assay pages

J-yeast-display Yeast display

K-BLI-DEMO-PDL1-001 BLI

K-BLI-DEMO-PDL1-002 BLI

K-BLI-DEMO-PDL1-003 BLI

K-BLI-DEMO-PDL1-004 BLI

K-BLI-DEMO-PDL1-005 BLI

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Where it stands

K

PDL1-R1 · Active

Human PD-L1 IgV domain, GFCC′ face overlapping atezolizumab, 55-65 aa, human/mouse cross-reactive, 24 in the first order.

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Your turn

Start a campaign

Pick a target and the campaign page carries the rest.

Tutorial